The Science

NutraStem® Science "Based on Science not Science Fiction"

NutraStem®’s patented formula has been scientifically studied and has been proven to support the normal growth and function of adult stem cells. Research demonstrates that the combination of ingredients found in NutraStem® helps to promote stem cell nutrition and the natural growth of adult stem cell populations commonly found within the body. NutraStem® contains a blend of wild blueberry extract, Vitamin D3, green tea extract and carnosine.

In research that was published in a peer-reviewed journal article we examined the effects of several natural compounds that have been shown to promote healing for their ability to increase the health and normal division of human adult stem cells when grown in culture.

• We exposed three different types of adult stem cells, which are commonly found within the body, to natural compounds that are known to promote healing and measured the growth of these cells (Figure 1).
• Our results demonstrate a significant growth in the cells exposed to blueberry extract, green tea, catechin, carnosine and Vitamin D3, and that a specific combination of these ingredients at certain doses could promote even more growth of these stem cells.
• The control that was used to compare these effects was human granulocyte-macrophage colony-stimulating factor (hGM-CSF) which is used in humans to increase the numbers of stem cells in the blood.

Figure 1
Bickford et al., 2006;
Stem Cells and Development
HGM-CSF-control
BB-blueberry
CH-catechin
Ca-carosine
GT-green tea
D3-vitamin D3

When the ingredients in NutraStem® are combined together there is a synergistic effect, which means that our proprietary formulation has an effect larger than simply adding together the effects of the individual ingredients (Figure 2) Bickford et al., 2006; Stem Cells and Development.

Figure 2
Bickford et al., 2006;
Stem Cells and Development

NutraStem® also has been shown to protect bone marrow stem cells from oxidative insult as demonstrated in an in vivo study in the journal of Rejuvenation Research.

Animals were fed NutraStem® in their food for 2 weeks, then the bone marrow was removed and grown in culture, and exposed to H202 (oxidative insult) which resulted in cell death in control animals that did not ingest NutraStem®.

Bone marrow cells from animals that ingested NutraStem® at the high dose showed a decrease in cell death (greater than 50%) and at the low dose (greater than 25%) compared to control animals (Figure 3).

Figure 3
Shytle et al., 2007;
Rejuvenation Research
Low dose= 13.5mg/kg/day
High dose= 135mg/kg/day

In addition, NutraStem® has displayed protection against ischemic stroke in an animal model as demonstrated in the journal of Rejuvenation Research (Yasuhara et al., 2008).

One group of animals were given a daily oral dose of NutraStem® (NT-020), while a second group of animals were given vehicle (control solution not containing NT-020), for two weeks. On Day 14 all animals underwent stroke surgery; middle cerebral artery occlusion (MCAO)

Both groups of animals were subjected to behavioral testing before stroke and on Day 14 post-stroke. The NutraStem®-treated stroke animals showed significantly less abnormalities in neurologic (24.4% reduction) and motor asymmetry (11.8% reduction) tests compared to vehicle-treated stroke animals (Figure 4).

Figure 4
Yasuhara et al., 2008;
Rejuvenation Research

Moreover, a significant 75% decrease in the stroke glial scar area (scar tissue resulting from stroke) was observed in the ischemic striatum of the NutraStem®-treated stroke animals compared to that of the vehicle-treated stroke animals (Figure 5).

Figure 5
Yasuhara et al., 2008;
Rejuvenation Research

Quantitative analysis also revealed that NutraStem® induced neural differentiation, with an increased tendency towards neuronal over glial lineage (Figure 6A & B).

Figure 6 A&B
Yasuhara et al., 2008;
Rejuvenation Research